LOCUS       IS26-tetA(D)-IS26_unit  5166 bp    DNA     linear       03-OCT-2020
DEFINITION  Enterobacter cloacae.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Unclassified.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of IS26-tetA(D)-IS26 unit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-OCT-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
     repeat_region   complement(1..14)
                     /gene="IRL_IS26"
     CDS             complement(53..757)
                     /gene="tnpA"
                     /codon_start=1
                     /product="IS26 transposase"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     CDS             complement(769..1425)
                     /gene="tetR(D)"
                     /codon_start=1
                     /product="Tetracycline repressor protein TetR, class D"
                     /translation="MARLNRESVIDAALELLNETGIDGLTTRKLAQKLGIEQPTLYWH
                     VKNKRALLDALAVEILARHHDYSLPAAGESWQSFLRNNAMSFRRALLRYRDGAKVHLG
                     TRPDEKQYDTVETQLRFMTENGFSLRDGLYAISAVSHFTLGAVLEQQEHTAALTDRPA
                     APDENLPPLLREALQIMDSDDGEQAFLHGLESLIRGFEVQLTALLQIVGGDKLIIPFC
                     "
     repeat_region   complement(807..820)
                     /gene="IRR_IS26"
     CDS             1521..2705
                     /gene="tetA(D)"
                     /codon_start=1
                     /product="Tetracycline efflux protein TetA, class D"
                     /translation="MNKPAVIALVITLLDAMGIGLIMPVLPSLLREYLPEADVANHYG
                     ILLALYAVMQVCFAPLLGRWSDKLGRRPVLLLSLAGAAFDYTLLALSNVLWMLYLGRI
                     ISGITGATGAVAASVVADSTAVSERTAWFGRLGAAFGAGLIAGPAIGGLAGDISPHLP
                     FVIAAILNACTFLMVFFIFKPAVQTEEKPAEQKQESAGISFITLLKPLALLLFVFFTA
                     QLIGQIPATVWVLFTESRFAWDSAAVGFSLAGLGAMHALFQAVVAGALAKRLSEKTII
                     FAGFIADATAFLLMSAITSGWMVYPVLILLAGGGIALPALQGIISAGASAANQGKLQG
                     VLVSLTNLTGVAGPLLFAFIFSQTQQSADGTVWLIGTALYGLLLAICLLIRKPAPVAA
                     TC"
     CDS             2770..3909
                     /gene="adhC"
                     /codon_start=1
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /translation="MISLRHRGTVMKSRAAVAFGPGLPLEIVEIDVAPPKKGEVLVKI
                     SHTGVCHTDAYTLSGDDPEGLFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAEC
                     GECDFCTSGKTNLCVAVRETQGKGVMPDGTSRFSYNGQPLYHYMGCSTFSEYTVVAEV
                     SLAKINPQANAEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGGIGLAVVQGARQ
                     AKAGRIFAIDTNPSKFELAKQFGATDCINPNDYDKPVQQVLVEMTKWGVDHTFECIGN
                     VNVMRSALESAHRGWGQSVIIGVAGAGKEISTRPFQLVTGRVWKGTAFGGVKGRTQLP
                     GMVEDAMSGKIELAPFVTHTMELDKINEAFDLMHDGKSIRTVIHY"
     CDS             3931..4365
                     /gene="orf435"
                     /codon_start=1
                     /product="Orf435 Protein"
                     /translation="MERIEHHVCFGGSQEVWRHHSAVTGTPMTFSVFLPPQAKTEKCP
                     VLYWLSGLTCNEQNFITKAGAQRYAAEHGLVIVAPDTSPRGSQVADDKEYDLGQGAGF
                     YVNATQEPWKAHYQMYDYILNELHTLVSGHFGTSEKRALLQS"
     repeat_region   complement(4347..4360)
                     /gene="IRL_IS26"
     CDS             complement(4399..5103)
                     /gene="tnpA"
                     /codon_start=1
                     /product="IS26 transposase"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     repeat_region   complement(5153..5166)
                     /gene="IRR_IS26"
ORIGIN      
        1 ggcactgttg caaagttagc gatgaggcag ccttttgtct tattcaaagg ccttacattt
       61 caaaaactct gcttaccagg cgcatttcgc ccaggggatc accataataa aatgctgagg
      121 cctggccttt gcgtagtgca cgcatcacct caataccttt gatggtggcg taagccgtct
      181 tcatggattt aaatcccagc gtggcgccga ttatccgttt cagtttgcca tgatcgcatt
      241 caatcacgtt gttccggtac ttaatctgtc ggtgttcaac gtcagacggg caccggcctt
      301 cgcgtttgag cagagcaagc gcgcgaccat aggcgggcgc tttatccgtg ttgatgaatc
      361 gcgggatctg ccacttcttc acgttgttga ggattttacc cagaaaccgg tatgcagctt
      421 tgctgttacg acgggaggag agataaaaat cgacagtgcg gccccggctg tcgacggccc
      481 ggtacagata cgcccagcgg ccattgacct tcacgtaggt ttcatccatg tgccacgggc
      541 aaagatcgga agggttacgc cagtaccagc gcagccgttt ttccatttca ggcgcataac
      601 gctgaaccca gcggtaaatc gtggagtgat cgacattcac tccgcgttca gccagcatct
      661 cctgcagctc acggtaactg atgccgtatt tgcagtacca gcgtacggcc cacagaatga
      721 tgtcacgctg aaaatgccgg cctttgaatg ggttcatgtg cagctccatc agcaaaaggg
      781 gatgataagt ttatcaccac cgactatttg caacagtgcc gtaagctgca cctcaaaccc
      841 ccggatcagg ctctccaggc catgcagaaa ggcctgctca ccatcatcac tgtccataat
      901 ctgcagcgct tcccgcaata gcggcggcag gttttcgtcc ggtgctgcag ggcggtcggt
      961 cagggcggca gtatgctcct gctgctccag tacggcacca agggtaaaat gactgaccgc
     1021 tgaaatcgca tataacccgt cgcgcagtga aaagccgttt tctgtcataa agcgtaactg
     1081 ggtttccacc gtatcatact gtttttcatc agggcgggtg ccgaggtgca cttttgcccc
     1141 gtcacggtaa cgcagcagcg cccggcggaa actcattgca ttattgcgca gaaatgactg
     1201 ccaggattcc cccgccgcag gcagtgaata atcatgatga cgcgccagga tctccaccgc
     1261 cagcgcatcc agtaacgccc gtttattttt cacatgccag taaagtgtcg gctgttctat
     1321 tcccagcttc tgcgccagct tgcgggtcgt cagcccgtca atccctgtct cattcagcag
     1381 ttccagtgcc gcatcaataa ccgattctct gttcagccgt gccatgaccg tctcctccga
     1441 ttttcagatt gacactctat cattgatagg gatatattcc aactctatca atgataggga
     1501 attaacacag gatctgaaaa atgaataaac ccgctgtcat cgcgctggtg attacactgc
     1561 tggacgcgat gggaattggt ctgatcatgc cggtattacc gtcactgctg cgggaatatc
     1621 tcccggaagc ggatgtggca aaccattacg gcattctgct ggcgctgtat gcggtgatgc
     1681 aggtctgttt tgctccgctg ctgggcagat ggtcagataa gctggggcgc agaccggtgc
     1741 tgctgttatc cctggcgggt gccgcgtttg attacacact gctggcactg tccaatgtgc
     1801 tgtggatgtt gtatctcggg cggattatct ccgggatcac tggtgccacc ggcgcggttg
     1861 cggcttcggt agtggcggac agcacggcgg tcagcgagcg taccgcctgg ttcggccgtc
     1921 tcggtgcggc ctttggtgcc gggctgattg ccgggccggc tatcggcgga ctggcggggg
     1981 atatctcacc gcatctgccg tttgtcattg cggcaatact gaatgcctgc acctttctga
     2041 tggtcttttt tatctttaaa ccggcggtac agacagaaga aaaaccggcg gagcagaaac
     2101 aagaaagcgc aggtatcagc tttatcacac tgcttaaacc tctggcgctg ttgctgtttg
     2161 tcttttttac cgcgcagctt atcgggcaga tcccggccac tgtctgggta ttgtttacgg
     2221 agagccgctt tgcctgggac agcgcggcgg tcggtttttc actggcggga ctcggggcga
     2281 tgcatgcact gtttcaggcg gtggttgccg gggcgctggc aaaacggctg agtgagaaaa
     2341 ccattatttt cgccggattt attgccgatg ccaccgcgtt tttactgatg tctgctatca
     2401 cttccggatg gatggtgtat ccggtcctga tcctgctggc aggcggcgga attgcactgc
     2461 ctgcattgca gggcattatc tctgccgggg catcggcggc aaatcaggga aaactacagg
     2521 gtgtgctggt cagcctgacc aatctgaccg gcgtggcggg cccgctgctg tttgctttta
     2581 ttttcagtca gacacagcag agtgcggacg gtacggtgtg gctgattggc acggcactgt
     2641 acggtctgct gctggcaatc tgtctgctga tcagaaaacc ggcaccggtg gcggccacct
     2701 gctgaccggc gctatacagg cagcgctgtt gtgcggtatg gtggacggca ttcgtgctta
     2761 tcgcctgcca tgataagcct cagacacaga ggaaccgtta tgaaatcacg cgcagcagtc
     2821 gccttcgggc cgggtttacc ccttgaaatt gttgaaattg atgtggcacc gccgaagaaa
     2881 ggtgaagtgc tggtgaaaat cagtcacacc ggggtatgcc ataccgatgc ctacacgctt
     2941 tccggtgatg atccggaagg tctgttcccg gtggtgctcg gccatgaagg tgccggggtg
     3001 gtggttgagg tcggtgaggg cgtgaccagt gtgaaaccgg gcgaccatgt gatcccgctg
     3061 tacaccgccg agtgcggcga gtgtgatttc tgtacctccg gtaaaaccaa cctctgtgtt
     3121 gccgtccgcg aaacccaggg caaaggcgtg atgccggacg gcaccagccg tttttcgtat
     3181 aacggtcagc cgctctatca ctatatgggc tgctcgacat tcagcgaata taccgttgtg
     3241 gcggaagtat cactggcgaa aatcaatccg caggccaatg cggagcaggt ttgcctgctc
     3301 ggctgcggcg tgaccaccgg gatcggcgcg gtacacaata ccgcgaaggt acaggaaggg
     3361 gattctgtgg cggtattcgg cctcggcggt atcgggctgg ccgtggtgca gggcgcacgt
     3421 caggcaaaag cggggcgcat ctttgccatc gataccaatc catccaagtt tgagctggca
     3481 aaacagttcg gcgccacaga ctgcattaat cccaatgatt atgacaaacc tgttcagcag
     3541 gtgctggtcg aaatgaccaa atggggtgtg gatcatacct ttgaatgtat cggcaatgtc
     3601 aatgtgatgc ggtcggcgct ggaaagtgcg caccgtggct gggggcagtc ggtaattatc
     3661 ggggtggcgg gtgccggaaa agagatttca acccgtccgt tccagctggt gaccggccgg
     3721 gtctggaaag gcacggcgtt cggcggcgtg aaagggcgta cccagttgcc gggaatggtg
     3781 gaagatgcca tgagcggcaa aatcgagctg gcgccgtttg tcacgcacac catggaactg
     3841 gataaaatca atgaagcttt tgatttgatg cacgacggca aatccatccg cacagtcatt
     3901 cattactgat aacgtattca gggagatact atggaacgga ttgaacatca cgtctgtttc
     3961 ggcggcagcc aggaagtctg gcgtcaccac tctgcggtga ccggcacgcc gatgactttt
     4021 tccgtgtttc tgccgccgca ggcaaaaaca gagaaatgtc ctgtgctgta ctggctttcc
     4081 gggctgacct gcaacgagca gaattttatc accaaagccg gggcacagcg ttacgcagcg
     4141 gaacacggcc tggtgattgt cgcgccggat accagtccgc gcggcagtca ggtggcggat
     4201 gataaagagt acgatctcgg ccagggtgcc gggttttatg tcaatgccac acaggagccg
     4261 tggaaagcgc attatcagat gtatgattat attctgaatg agctgcacac cctggtaagc
     4321 gggcatttcg gcacatcgga aaaacgggca ctgttgcaaa gttagcgatg aggcagcctt
     4381 ttgtcttatt caaaggcctt acatttcaaa aactctgctt accaggcgca tttcgcccag
     4441 gggatcacca taataaaatg ctgaggcctg gcctttgcgt agtgcacgca tcacctcaat
     4501 acctttgatg gtggcgtaag ccgtcttcat ggatttaaat cccagcgtgg cgccgattat
     4561 ccgtttcagt ttgccatgat cgcattcaat cacgttgttc cggtacttaa tctgtcggtg
     4621 ttcaacgtca gacgggcacc ggccttcgcg tttgagcaga gcaagcgcgc gaccataggc
     4681 gggcgcttta tccgtgttga tgaatcgcgg gatctgccac ttcttcacgt tgttgaggat
     4741 tttacccaga aaccggtatg cagctttgct gttacgacgg gaggagagat aaaaatcgac
     4801 agtgcggccc cggctgtcga cggcccggta cagatacgcc cagcggccat tgaccttcac
     4861 gtaggtttca tccatgtgcc acgggcaaag atcggaaggg ttacgccagt accagcgcag
     4921 ccgtttttcc atttcaggcg cataacgctg aacccagcgg taaatcgtgg agtgatcgac
     4981 attcactccg cgttcagcca gcatctcctg cagctcacgg taactgatgc cgtatttgca
     5041 gtaccagcgt acggcccaca gaatgatgtc acgctgaaaa tgccggcctt tgaatgggtt
     5101 catgtgcagc tccatcagca aaaggggatg ataagtttat caccaccgac tatttgcaac
     5161 agtgcc
//
